Adenovirus DNA binding protein: helix destabilising properties
نویسندگان
چکیده
منابع مشابه
NikR is a ribbon-helix-helix DNA-binding protein.
Escherichia coli NikR, a repressor with homologs in other bacteria and archaea, was identified as a potential new member of the ribbon-helix-helix (beta-alpha-alpha) family of transcription factors in profile based sequence searches and in structure prediction experiments. Biophysical and biochemical characterization of the N-terminal domain of NikR show that it has many features expected of a ...
متن کاملDNA binding properties of the adenovirus DNA replication priming protein pTP.
The precursor terminal protein pTP is the primer for the initiation of adenovirus (Ad) DNA replication and forms a heterodimer with Ad DNA polymerase (pol). Pol can couple dCTP to pTP directed by the fourth nucleotide of the viral genome template strand in the absence of other replication proteins, which suggests that pTP/pol binding destabilizes the origin or stabilizes an unwound state. We an...
متن کامل- turn - helix ; DNA - binding proteins )
The Saccharomyces cerevisiae CDC43 gene product is involved in establishing cell polarity during the cell-division cycle. When grown at restrictive temperatures, temperature-sensitive cdc43 mutants are unable to form buds and display delocalized cell-surface deposition [Adams et al., J. Cell Biol. (1990) in press]. We have isolated a cdc43-complementing plasmid from a yeast genomic-DNA library ...
متن کاملStructure-function relationships of the adenovirus DNA-binding protein.
The M, = 72,000 adenovirus-coded DNA-binding protein is required for viral DNA replication and is involved in virus transcription and transformation. This protein, as isolated from infected cells, is a phosphoprotein with extensive charge heterogeneity. Between 9 and 11 distinct charged species of this protein were detected by isoelectric focusing. Treatment of the protein with alkaline phospha...
متن کاملBiochemical analysis of adenovirus type 5 DNA-binding protein mutants.
We previously reported the isolation and functional characterization of seven adenovirus type 5 (Ad5) DNA-binding protein (DBP) point mutants (Quinn, C. O., and Kitchingman, G. R. (1986) J. Virol. 60, 653-661). Six of the seven mutants were defective in their ability to help adeno-associated virus replicate its DNA. To determine the level at which the mutations affect this function of the DBP, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1994
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/22.5.742